Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models

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dc.contributor.authorGülnar, Birgül
dc.contributor.authorCanatar, İpek
dc.contributor.authorÖzdaş, Sibel
dc.date.accessioned2025-01-06T17:30:28Z
dc.date.available2025-01-06T17:30:28Z
dc.date.issued2023
dc.description.abstractSince obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this study, it was aimed to evaluate the antiadipogenic and antiobesogenic efficacy of pterostilbene a natural phenolic compound in 3T3-L1 cells. The mature 3T3-L1 adipocytes were exposed to pterostilbene at different concentrations and half-maximum inhibitory concentrations (IC50 ) were determined by MTT assay. Oil-Red-O staining was applied to determine lipid accumulation. Phase contrast microscopy, Giemsa, F-actin and DAPI staining were applied to examine the efficacy of pterostilbene on the morphology of 3T3-L1 adipocyte cells. Moreover, expressions of adinopectin and glucose transporter-4 (Glut-4) in relation to insulin resistance were evaluated using immunofluorescent staining and qRT-PCR. Pterostilbene caused no significant cytotoxicity towards preadipocytes at concentrations ?7.5 ?M and the percentage of viable cells remained above about 86% for 24 h, 48 h and 72 h (p > 0.05). Therefore, pterostilbene treatment at 5 and 7.5 ?M was used in the subsequent experiments as safe dosages. In addition, it was observed that pterostilbene treatment reduced lipid accumulation in adipocyte differentiation. Adipocytes treated with a dose of 7.5 µM for 14 days showed less intense lipid deposition and a more spindle-like morphology compared to the adipocytes treated with a dose of 5 µM. Especially on the 14th day, actin filaments were filamentous in adipocytes treated with pterostilbene 7.5 µM compared to the adipocytes treated with a dose of 5 µM; the filaments were similarly oriented as in preadipocytes, and chromatin condensation was observed to be quite high. Our data suggests that the pterostilbene supplementation may help weight control and the antiadipogenic and that antiobesogenic activity is mediated in part by reduction of lipid accumulation and induction of Glut-4 and Adiponectin levels. © TÜBİTAK.
dc.description.sponsorshipAdama Science and Technology University, ASTU, (21332003)
dc.identifier.doi10.55730/1300-0152.2649
dc.identifier.endpage157
dc.identifier.issn1300-0152
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85159185395
dc.identifier.scopusqualityQ1
dc.identifier.startpage141
dc.identifier.trdizinid1180110
dc.identifier.urihttps://doi.org/10.55730/1300-0152.2649
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1180110
dc.identifier.urihttps://hdl.handle.net/20.500.14669/1643
dc.identifier.volume47
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTUBITAK
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subject3T3-L1
dc.subjectadipogenesis
dc.subjectadiponectin
dc.subjectAntiadipogenic
dc.subjectantiobesogenic
dc.subjectdifferentiation
dc.subjectglucose transporter-4
dc.subjectpterostilbene
dc.titleAntiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
dc.typeArticle

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