Yazar "Zhu, Lin" seçeneğine göre listele

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    Öğe
    Activated expression of EsHD1 enhances drought tolerance in tobacco plants via mitigation of reactive oxygen species-mediated membrane damage
    (2015) Zhou, Cheng; Ma, Zhongyou; Zhu, Lin; Guo, Jiansheng; Cuı, Xianghuan; Zhu, Jian; Wang, Jianfei
    It is well documented that the homeodomain-leucine zipper (HD-Zip) transcription factors play diverse roles during plant growth and development. The Class I HD-Zip genes are shown to be involved in the regulation of abiotic stress responses and tolerance. Herein, a novel Class I HD-Zip gene, EsHD1, was isolated from Eutrema salsugineum, and an investigation was performed of its physiological functions in response to drought stress. The analyses of gene expression profiles revealed that the EsHD1 transcripts quickly accumulated upon exposure to various abiotic stress conditions or abscisic acid treatments. Furthermore, the overexpression of EsHD1 in tobacco plants conferred improved tolerance to drought stress. The EsHD1-overexpressing lines had lower levels of reactive oxygen species (ROS), ion leakage, and malondialdehyde, but they manifested higher activities of antioxidant enzymes and the transcription of pathogenic-related genes as compared to wild-type plants under drought stress conditions. Therefore, our findings demonstrated that EsHD1 positively regulated plant drought tolerance via alleviation of ROS-mediated membrane damage.
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    Öğe
    Heterologous expression of EsABA1 enhances salt tolerance with increased accumulation of endogenous ABA in transgenic tobacco
    (2014) Sun, Yujun; Zhu, Lin; Guo, Jiansheng; Zhou, Cheng
    Salt stress can trigger several physiological responses in plants such as increased accumulation of ABA and antioxidantenzyme activities, which have been closely associated with the tolerance of plants to salt stress. In the present study a novel zeaxanthinepoxidase gene, designated EsABA1 , was isolated from Eutrema salsugineum. EsABA1 contained a full-length open reading frame(ORF) of 2001 bp, encoding 666 amino acids. Transcription of EsABA1 rapidly accumulated afer exposure to various abiotic stresses,suggesting that the EsABA1 gene was involved in multiple stress responses and tolerance. To further investigate the roles of EsABA1under salt-stress conditions, transgenic tobacco lines that ectopically overexpressed EsABA1 were obtained. Tese transgenic linesexhibited more tolerance to salt stress than wild-type (WT) plants, and overexpression of EsABA1 increased the contents of ABA andproline as compared to WT plants under salt-stress conditions. Moreover, the expression levels of stress responsive genes and activitiesof antioxidant enzymes were much higher in the EsABA1-overexpressing lines than in WT plants. Terefore, our fndings indicated thatthe elevated expression of EsABA1 enhanced the tolerance of transgenic tobacco plants to high salinity stress.