Heterologous expression of EsABA1 enhances salt tolerance with increased accumulation of endogenous ABA in transgenic tobacco

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Tarih

2014

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Salt stress can trigger several physiological responses in plants such as increased accumulation of ABA and antioxidantenzyme activities, which have been closely associated with the tolerance of plants to salt stress. In the present study a novel zeaxanthinepoxidase gene, designated EsABA1 , was isolated from Eutrema salsugineum. EsABA1 contained a full-length open reading frame(ORF) of 2001 bp, encoding 666 amino acids. Transcription of EsABA1 rapidly accumulated afer exposure to various abiotic stresses,suggesting that the EsABA1 gene was involved in multiple stress responses and tolerance. To further investigate the roles of EsABA1under salt-stress conditions, transgenic tobacco lines that ectopically overexpressed EsABA1 were obtained. Tese transgenic linesexhibited more tolerance to salt stress than wild-type (WT) plants, and overexpression of EsABA1 increased the contents of ABA andproline as compared to WT plants under salt-stress conditions. Moreover, the expression levels of stress responsive genes and activitiesof antioxidant enzymes were much higher in the EsABA1-overexpressing lines than in WT plants. Terefore, our fndings indicated thatthe elevated expression of EsABA1 enhanced the tolerance of transgenic tobacco plants to high salinity stress.

Açıklama

Anahtar Kelimeler

Kaynak

Turkish Journal of Botany

WoS Q Değeri

Scopus Q Değeri

Cilt

38

Sayı

6

Künye