Yazar "Usta, O. Berk" seçeneğine göre listele

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    Evaluation of drug carrier hepatotoxicity using primary cell culture models
    (Elsevier, 2023) Kibar, Gunes; Dutta, Subhadeep; Rege, Kaushal; Usta, O. Berk
    This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concen-trations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 mu g/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physi-cochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.(c) 2023 Published by Elsevier Inc.
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    Öğe
    Novel 3D-Printed Microfluidic Magnetic Platform for Rapid DNA Isolation
    (Amer Chemical Soc, 2024) Kibar, Gunes; Sariarslan, Busra; Doganay, Serkan; Yildiz, Gokay; Usta, O. Berk; Cetin, Barbaros
    This study presents a novel miniaturized device as a 3D-printed microfluidic magnetic platform specifically designed to manipulate magnetic microparticles in a microfluidic chip for rapid deoxyribonucleic acid (DNA) isolation. The novel design enables the movement of the magnetic particles in the same or opposite directions with the flow or suspends them in continuous flow. A computational model was developed to assess the effectiveness of the magnetic manipulation of the particles. Superparamagnetic monodisperse silica particles synthesized in-house are utilized for the isolation of fish sperm DNA and human placenta DNA. It was demonstrated that the proposed platform can perform DNA isolation within 10 min with an isolation efficiency of 50% at optimum operating conditions.