Yazar "Baydemir Pesint, Gozde" seçeneğine göre listele

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    Determination of mold contamination using ergosterol imprinted particles
    (Wiley, 2021) Oktay Basegmez, Hatice Imge; Baydemir Pesint, Gozde; Nergiz, Mustafa; Zenger, Okan
    Ergosterol is a key biochemical marker for fungal mycelial growth. In this study, molecularly ergosterol imprinted particles (Erg-MIPs) were newly synthesized for the selective detection of ergosterol in mold samples. Erg-MIPs were characterized via scanning electron microscopy, swelling studies, and surface area measurements. Maximum selective ergosterol adsorption achieved as 28.50 mg/g Erg-MIP. Selectivity studies showed that Erg-MIPs adsorbed Erg 2.01 and 3.27 times higher than that of cholesterol and stigmasterol, respectively. Erg adsorption fromAspergillus nigerwas found as 23.87 mg/g. Reusability of Erg-MIPs was studied and decrease in Erg adsorption capacity of the particles was negligible (3%). Erg-MIPs are good affinity materials for the selective Erg detection from food samples, prior to use in food industry.
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    Molecularly imprinted composite discs for transferrin recognition
    (Taylor & Francis Inc, 2022) Aylaz, Gulgun; Zenger, Okan; Baydemir Pesint, Gozde; Andac, Muge
    Human Transferrin (HTr) imprinted composite cryogel (HTr-MIPCC) discs were synthesized with distinctive structure and increased adsorption capacity and specificity for HTr by combining the advantages of the surface imprinting technique. HEMA-based cryogel was embedded with HTr-surface imprinted particles. Thanks to the imprinted particles embedding, both the higher surface area and specific recognition are provided. The structure of the HTr-MIPCC was characterized by FTIR Spectrometer, SEM, swelling studies, flow dynamics, and surface area measurements. The addition of surface imprinted particles into the cryogel disc has resulted in an increased surface area, enhancing the capacity of composite cryogel for HTr adsorption up to 10.96 mg/g and gel fractionation yields reached up to 83%. Selectivity studies were carried out against HIgG and HSA as competitors. The HTr-MIPCC discs were packaged into an FPLC column for selective depletion of HTr. It was calculated that the HTr-MIPCC is 1.071 and 1.062 times selective for HTr than HSA and HIgG, respectively. According to FPLC studies HTr-MIPCC can be considered as an excellent alternative for affinity matrixes used for the detection and selective recognition of HTr directly from human serum. HTr-MIPCC discs can be used several times without any noteworthy reduction in the adsorption capacity of HTr.
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    Monolithic hydrophobic cryogel columns for protein separation
    (Springer, 2022) Erzengin, Mahmut; Baydemir Pesint, Gozde; Zenger, Okan; Odabasi, Mehmet
    The present study was conducted for the synthesis of a novel supermacroporous monolithic hydrophobic adsorbent for lysozyme (Lyz) selected as a model protein from aqueous solution. After preparation of poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-(l)-tyrosine methyl ester) monolithic cryogel column, 1-naphthylamine was covalently attached, and the prepared column was abbreviated as NA-Mcc. Scanning electron microscopy, Fourier transform infrared spectroscopy and Brunauer, Emmett and Teller device were utilized for the morphology, functional groups and surface area measurements of the column. Effects of several parameters including Lyz content of the adsorption solution, pH of the medium used, ambient temperature, type of salt and flow rate on the adsorption capacity of the polymeric material were examined in continuous operation. The maximum value achieved for Lyz adsorption from aqueous phase was found to be 105.8 mg/g in phosphate buffer. In addition, NA-Mcc was investigated in terms of reusability, and it was demonstrated that there is no significant change in the adsorption properties of prepared monolithic hydrophobic cryogels after 30 adsorption-desorption cycles.
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    Neopterin imprinted nanofilm decorated SPR sensors: Sensitive neopterin detection from human serum
    (Springer, 2024) Eren Yungevis, Burcu; Baydemir Pesint, Gozde
    Neopterin (Np) is a valuable marker for monitoring infectious diseases and predicting prognosis. This study developed Np-imprinted surface plasmon resonance (SPR) sensors, specifically designed to detect Np in human serum. Characterization of the Np-imprinted (Np-MIP) and non-imprinted (NIP) SPR sensors was carried out using various techniques. The contact angle measurements revealed increased hydrophilicity of the gold sensor with the nanofilm covering, reducing the contact angles from 60.5 degrees to 45.87 degrees and 32.7 degrees for NIP and Np-MIP SPR sensors, respectively. The nanofilm thickness was determined as approximately 14 nm through AFM studies. Real-time Np detection was successfully achieved with the Np-MIP SPR sensors at different concentrations. The selectivity of Np-MIP was confirmed by testing against pterin and glucose, with Np recognition being 11 and 8.25 times higher than those of pterin and glucose, respectively. Np detection from human serum yielded a detection limit of 3 ng/mL. Furthermore, the synthesized Np-MIP SPR sensors demonstrated reusability over 10 cycles without any loss in Np binding capacity. These findings indicate that Np-MIP SPR sensors possess selective detection capabilities for Np in aqueous solutions and human serum.