Antibacterial and Antioxidant Activity of Isoflavans from the Roots of Rhynchosia ferruginea and In Silico Study on DNA Gyrase and Human Peroxiredoxin

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dc.contributor.authorHussien, Kalid
dc.contributor.authorEswaramoorthy, Rajalakshmanan
dc.contributor.authorMelaku, Yadessa
dc.contributor.authorAnnisa, Milkyas Endale
dc.date.accessioned2025-01-06T17:22:51Z
dc.date.available2025-01-06T17:22:51Z
dc.date.issued2021
dc.departmentAdana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi
dc.description.abstractRhynchosia ferruginea (Udusalim, Afan Oromo) is a medicinal plant traditionally used to treat skin infection, intestinal problems and amoebiasis. Silica gel chromatographic separation of dichloromethane/methanol (1:1) roots extract yielded isoflavan (1), isoflavene (2) and 1, 3-dilinoleoyl-2- stearoylglycerol (3), reported herein for the first time from the genus. Antibacterial activity was examined using disc diffusion method against E. coli, S. aureus, P. aeruginosa and S. pyogenes. AutoDoc vina 4.2 software was used for molecular docking analysis of compounds against human peroxiredoxin 5 and DNA gyrase B enzymes. Isoflavan (1) displayed zone of inhibition of 9.67 ± 0.58 mm and 10.67 ± 0.58 mm whereas isoflavene (2) showed 10.33 ± 1.15 mm and 10 ± 1.00 mm against E. coli and S. aureus, respectively, compared to ciprofloxacin (15.67±0.58 mm for both strains). DPPH radical scavenging assay of the dichloromethane/methanol (1:1) roots extract and isoflavan (1) exhibited better radical scavenging activity with $IC_{50}$ value of 17.7 and 32, respectively. Molecular docking analysis revealed that 1 and 2 exhibited similar binding affinity of -7.4 kcal/mol compared to ciprofloxacin (-7.3 kcal/mol). In silico analysis against human peroxiredoxin 5 (PDB ID: 1HD2) revealed minimum binding affinity of - 3.7 and - 2.0 kcal/mol for compounds 1 and 2, respectively, compared to ascorbic acid (-4.9 kcal/mol). The in vitro antibacterial and antioxidant activity of compounds 1 and 2 suggest the potential use of these compounds as drug lead candidates which corroborate with the traditional uses of the roots of R. ferruginea.
dc.identifier.doi10.21448/ijsm.962120
dc.identifier.endpage336
dc.identifier.issn2148-6905
dc.identifier.issue4
dc.identifier.startpage321
dc.identifier.trdizinid1148442
dc.identifier.urihttps://doi.org/10.21448/ijsm.962120
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1148442
dc.identifier.urihttps://hdl.handle.net/20.500.14669/506
dc.identifier.volume8
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofInternational Journal of Secondary Metabolite
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectMolecular docking
dc.subjectAntibacterial activity
dc.subjectRadical scavenging activity
dc.subjectRhynchosia ferruginea
dc.titleAntibacterial and Antioxidant Activity of Isoflavans from the Roots of Rhynchosia ferruginea and In Silico Study on DNA Gyrase and Human Peroxiredoxin
dc.typeArticle

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