IDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES

Basit Öğe Kaydı
dc.contributor.authorKadiroğlu, Pınar
dc.contributor.authorKorel, Figen
dc.contributor.authorCeylan, Çağatay
dc.date.accessioned2025-01-06T17:23:20Z
dc.date.available2025-01-06T17:23:20Z
dc.date.issued2019
dc.departmentAdana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi
dc.description.abstractThe problems on identification of Staphylococcus aureus isolates from cheese samples wereinvestigated by phenotypic and genotypic tests in this study. Among 207 Staphylococcus spp.isolated from 31 cheese samples, 23 isolates that were Gram positive, catalase and slide coagulasepositive, with 1 isolate that was latex agglutination test negative showed different phenotypicproperties. Polymerase chain reaction (PCR) and quantitative PCR (qPCR) analyses showed thatDNase test and target genes (nuc, coa) regarded as gold standard regions for S. aureus were notfound to be unique for identification of S. aureus. The toxin genes (SEA-SEE) were not detected byPCR. Antibiotic resistance profiles of S. aureus isolates demonstrated that two isolates were resistantto penicillin G. This study showed that the unique phenotypic and genotypic test was not adequatefor identification of S. aureus isolates. There was no correlation between the presence of the nucgene and toxin genes. The presence of nuc gene which was used for detection of S. aureus was alsofound to be present in other Staphylococcus isolates. As a conclusion, the results revealed thatbiochemical tests could lead to false positive results for identification of S. aureus. The presence ofnuc gene is not correlated with the presence of toxin genes.
dc.identifier.doi10.3153/FH19016
dc.identifier.endpage159
dc.identifier.issn2602-2834
dc.identifier.issue3
dc.identifier.startpage149
dc.identifier.trdizinid387996
dc.identifier.urihttps://doi.org/10.3153/FH19016
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/387996
dc.identifier.urihttps://hdl.handle.net/20.500.14669/724
dc.identifier.volume5
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofFood and Health
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241211
dc.subjectMikrobiyoloji
dc.subjectGıda Bilimi ve Teknolojisi
dc.titleIDENTIFICATION OF Staphylococcus aureus CHEESE ISOLATES WITH RESPECT TO VIRULENCE PROPERTIES, GENETIC RELATEDNESS AND ANTIBIOTIC RESISTANCE PROFILES
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu