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    Assessment of chitosan:gum tragacanth cryogels for tissue engineering applications
    (Wiley, 2022) Demir, Didem; Ugurlu, Muge Asik; Ceylan, Seda; Sakim, Burcu; Genc, Rukan; Bolgen, Nimet
    Gum tragacanth is one of the most widely used natural gums in food, medicine, cosmetics and personal care products, and its use as polysaccharide-based scaffolds in tissue engineering applications has attracted great attention in recent years. The fabrication of pure gum tragacanth as a scaffold poses many challenges because of the high viscosity, poor mechanical properties and repulsive interaction between the polyanions. To overcome these, facilitate the formation of scaffolds and improve their final properties, chitosan and gum tragacanth were used together as natural, biocompatible and biodegradable polysaccharides. The scaffolds based on chitosan and gum tragacanth were successfully fabricated through cryotropic gelation and were characterized using different chemical, morphological, mechanical and biocompatibility analyses. All cryogel scaffolds exhibited a porous structure with an average diameter of 96.56-30.21 mu m, exhibiting high liquid absorption capacity, appropriate mechanical stability and controlled degradation behavior. According to the biocompatibility results, mouse embryonic fibroblast cells adhered well to the scaffolds and achieved high viability. The results are also discussed in the light of their potential usefulness as a scaffold for tissue engineering applications. (c) 2022 Society of Industrial Chemistry.
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    Öğe
    Electrospun Composite Nanofibers Based on Poly (epsilon-Caprolactone) and Styrax Liquidus (Liquidambar orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results
    (Springer, 2022) Demir, Didem; Ozdemir, Sadin; Ceylan, Seda; Yalcin, M. Serkan; Sakim, Burcu; Bolgen, Nimet
    In this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mill. incorporated PCL fibrous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nanofibers were examined for their antioxidant activity, anti-biofilm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nanofibers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic fibroblast cells. The fibrous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.