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    Fatty acids, sterols and triglycerides composition of cold pressed oil from pomegranate seeds
    (Innovhub Ssi-Area Ssog, 2021) Kola, Osman; Parildi, Erva; Akkaya, Murat Reis; Ozcan, Bahri Devrim; Mamur, Emrah; Turker, Burcak
    Pomegranate seed oil (PSO) was obtained by cold pressing of seeds of pomegranate (Punica granatum L.) grown in Turkey. Total crude oil was obtained from pomegranate seeds on a 14.62% dry matter (DM) and it was determined to having a high content of polyunsaturated fatty acids (87.82%). The dominant fatty acid in pomegranate seeds was determined to be punicic acid (C18:3n-5; 9c, 11t, 13c) and it was found that it constitutes about 76.14% of the total fat content. It was observed that the total tocopherol content of PSO was 258.40 mg/100g and the main tocopherol was alpha-tocotrienol and the others were alpha-tocopherol (189.89 mg/100g) and gamma-tocopherol (53.14 mg/100g), respectively. The total sterol content of PSO was 930 mg/100g with a predominance of beta-sitosterol in our study and the beta-sterol content was 87.32%. The major triacylglycerols (TAG) in pomegranate seed oil were determined to be Stearic-Punicic-Punicic acid (SPuPu; 4.34%), Oleic-Punicic-Linoleic acid (OPuL; 1.10%) and Palmitic-Punicic-Linoleic acid (PPuL; 0.71%), respectively.
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    Fatty acids, sterols and triglycerides composition of cold pressed oil from pomegranate seeds
    (INNOVHUB - Stazioni Sperimentali per l'Industria S.r.l - Area Oli e Grassi, 2021) Kola, Osman; Parildi, Erva; Akkaya, Murat Reis; Özcan, Bahri Devrim; Mamur, Emrah; Türker, Burçak
    Pomegranate seed oil (PSO) was obtained by cold pressing of seeds of pomegranate (Puni-ca granatum L.) grown in Turkey. Total crude oil was obtained from pomegranate seeds on a 14.62% dry matter (DM) and it was determined to having a high content of polyunsaturated fatty acids (87.82%). The dominant fatty acid in pomegranate seeds was determined to be punicic acid (C18:3n-5; 9c, 11t, 13c) and it was found that it constitutes about 76.14% of the total fat content. It was observed that the total tocopherol content of PSO was 258.40 mg/100g and the main tocopherol was ?-tocotrienol and the others were ?-tocopherol (189.89 mg/100g) and ?-tocopherol (53.14 mg/100g), respectively. The total sterol content of PSO was 930 mg/100g with a predominance of ?-sitosterol in our study and the ?-sterol content was 87.32%. The major triacylglycerols (TAG) in pomegranate seed oil were determined to be Stearic-Punicic-Punicic acid (SPuPu; 4.34%), Oleic-Punicic-Lino-leic acid (OPuL; 1.10%) and Palmitic-Punicic-Linoleic acid (PPuL; 0.71%), respectively. © 2021, INNOVHUB - Stazioni Sperimentali per l'Industria S.r.l - Area Oli e Grassi. All rights reserved.
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    Fatty acids, triglycerides, tocol, and sterol contents of oils of some Moringa seed varieties
    (Innovhub Ssi-Area Ssog, 2023) Kola, Osman; Gokalp, Smeyye; Parildi, Erva; Ozkul, Neslihan; Akkaya, Murat Reis; Cetin, Ali Emrah
    Three moringa oliefera varieties (MOMAX 3, ODC and PKM-1) seeds were used in this study. The oil was extracted from the seeds of ODC, PKM1 and MOMAX 3 moringa varieties by cold press (CP) and the solvent extraction (SE) method. Fatty acids, triglycerides, tocols (tocopherols and tocotrienols) and sterol contents of moringa seed oils obtained by two different methods were determined and compared with one another. The crude oil yield was between 26.46 - 28.19% in solvent extraction and 24.20 - 26.30% in the cold press method. It was determined that the main fatty acid in moringa seed oils (ODC, PKM1 and MOMAX 3) was Oleic acid (63.31 - 70.04%). Other dominant fatty acids were determined to be palmitic acid (16:0) (5.59-7.26%), stearic acid (18:0) (5.37-5.89%), oleic acid (18:1 n9) (%63.31-70.04), arachidic acid (20:0) (2.67 - 3.71 %) and behenic acid (22:0) (3.82 5.73%). It was determined that the main triglyceride in moringa seed oils was triolein (000; 35.63 - 36.50%), the main sterol was [3-Sitosterol (39.04 - 42.11 %) and the main tocopherol was a-tocopherol (15.88 -18.91 pg/g).
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    Recombinant D-tagatose 3-epimerase production and converting fructose into allulose
    (Wiley, 2022) Parildi, Erva; Kola, Osman; Ozcan, Bahri Devrim; Akkaya, Murat Reis; Dikkaya, Elif
    In this study, D-tagatose 3-epimerase gene was amplified from Escherichia coli JM109 by PCR and re-cloned into E. coli JM109 using HincII digested pUC18 cloning vector. Digestion of recombinant plasmid by HincII and PCR amplification of the gene from recombinant plasmid produced 789 bp gene band on agarose gel which indicated the gene integration. The host cell was grown in a shaking incubator at 37 degrees C for 24 hr and then the optical density (OD) of the cells was measured in a spectrophotometer at 600 nm wavelengths. When the OD600 value reached 0.61, the protein expression was induced by the addition of 0.1 mM IPTG into the growth medium. Through His-select gel column purification, highly purified and stable DTE protein was produced. The conversion rate of D-fructose into D-allulose was determined by HPLC. The native and recombinant enzymes could effectively convert D-fructose into D-allulose with a turnover ratio of 20.76%. Novelty impact statement A recombinant enzyme, D-tagatose 3-epimerase, was produced and D-fructose was converted into D-allulose using this recombinant enzyme.