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    Detection of Potential Bacterial Pathogens and Aflatoxigenic Fungi from Grain Samples
    (2019) Mohamed, Hinda; Salauddi, Md.; Hossain, Khaled; Afro, Farzana
    Current research work was carried out for the detection of potential bacterial pathogen andaflatoxigenic fungi Aspergillus spp. from grain comprising [Rice (5), Maize (5), Wheat(5), Khessari dal (5) and Anchora dal (5)] were collected from 3 different local markets of DinajpurDistrict, Bangladesh. 15 bacterial isolates comprising 4 genera of bacteria were found from a totalof 25 samples. The isolated bacteria were Staphylococcus spp., Escherichia coli, Klebsiella spp.,Salmonella spp. with 16%, 28%, 16% and 16% prevalence respectively. Antibiogram studiesrevealed that overall effective drugs against isolated bacteria were Ciprofloxacin followed byGentamycin. But resistant drugs were Penicillin, Vancomycin, Erythromycin, Kanamycin, andAmoxicillin. The variation in the sensitivity of common antibiotic could be the result of extensiveand indiscriminate use of these antibiotics. Aspergillus spp. was isolated from 4-grain samples with16% prevalence. But aflatoxigenic Aspergillus spp. was isolated from 3 samples with 12%prevalence. From the wheat samples and maize, the aflatoxigenic fungus was isolated and theirprevalence in maize, wheat was 40% and 20% respectively. Their early detection can help to takepreventive measures to combat economic and health losses. The study showed that earlier detectionscan be made by simple traditional identifications using macro and micromorphological fungalfeatures rather than adopting the time and cost consuming molecular identification techniques.