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  • [ X ]
    Öğe
    Adaçayı Bitkisinin Osteosarkoma (U-2 OS) Hücre Hattındaki Sitotoksik Etkisinin İncelenmesi
    (2018) Göktürk, Dilek
    Adaçayı; Lamiaceae ailesine ait, aromatik, akdeniz kökenli bir bitkidir. Tüm dünyada, adaçayı, çeşitlihastalıklara deva bulmak ve hastalıklardan korunmak amacıyla yüzyıllardır geleneksel tedavidekullanılmaktadır. Adaçayı; sineol,borneol,rozmarinik asit, karnasol ve karnosik asitte dahil olmak üzerebir çok aktif bileşen ve esansiyal yağ içerir. Bu içeriği sayesinde antioksidan, antibakteriyel, anti-inflamatuar, antitümör gibi bir çok yararlı özelliğe sahip olmakla birlikte Alzheimer ve diyabet gibi çeşitlihastalıklarda da etkilidir. Bunun yanı sıra, Adaçayı bitkisinin bir çok kanser hücre hattı üzerinde etkiliolduğunu belirten birçok çalışma mevcuttur. Bu çalışmada ise adaçayı bitkisinin, birincil kemiktümörlerinin en malignant ve en yaygın görünen türü olan osteosarkoma hücreleri üzerindeki sitotoksiketkisinin incelenmesi amaçlanmıştır. Bu amaçla geleneksel tedaviden esinlenerek adaçayı infüzyonsolüsyonu olarak hazırlanmış ve U-2 OS osteosarkoma hücre hattı 3 gün boyunca çeşitli hacimoranlarında (1/1000, 1/100, 1/75, 1/50 ve 1/25 hacim/hacim) infüzyon solüsyonuna maruz bırakılmıştır.Bu süre sonunda hücre canlılığı nötral kırmızısı canlılık testi ile ölçülmüştür. Nötral kırmızısı canlılıktestine göre 1/1000, 1/100, 1/75, 1/50 and 1/25 hacim oranlarında verilen infüzyon solüsyonu U-2 OShücrelerinin canlılığını sırasıyla %3, %4, %26, %27 ve %29 oranında azaltmıştır. Elde edilen sonuçlar,adaçayı bitkisinin osteosarkoma hücreleri üzerinde sitotoksik etkisinin olduğunu desteklemektedir.
  • [ X ]
    Öğe
    Application of Multi-Criteria Decision Making Methods for Menu Selection
    (2024) İpek, Semih Latif; Göktürk, Dilek
    Nutritional information on menus can assist customers in making healthier eating choices. One technique being utilized to tackle the rise of overweight and obesity is the use of nutritional information on menus. Menu engineering strategies can be used to improve sales of generally healthier and higher margin items. For today's food and beverage companies, menu engineering has become essential. Companies must continually evaluate their menus in order to keep up with changing customer demands and the conditions of the competitive market. Menu engineering's core involves comparing the effectiveness of each menu. At this point, correct decision-making under numerous factors is thought to be a very challenging procedure. To evaluate alternatives according to many features, several Multi-Criteria Decision-Making (MCDM) approaches have been created. The main novelty of this paper is that four MCDM methods, including Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS), Fuzzy TOPSIS, VlseKriterijumska Optimizacija I Kompromisno Resenje (VIKOR), and Fuzzy VIKOR, are employed to evaluate menu options. Comparative analysis of MCDM methods is another contribution of this study. The process of evaluating and selecting healthier menu alternatives can become challenging and time-consuming. This study pointed out how crucial it is to conduct comparative analysis using various MCDA methods and to carefully determine the right ones when addressing the issue of selecting the best menu, taking into account the values of the criterion in fuzzy numbers.
  • [ X ]
    Öğe
    The Effect of Ascorbic Acid over the Etoposide- and Temozolomide-Mediated Cytotoxicity in Glioblastoma Cell Culture: A Molecular Study
    (2018) Göktürk, Dilek; Kelebek, Haşim; Ceylan, Seda; Yılmaz, Derviş Mansuri
    AIm: Glioblastoma (GBM) is one of the lethal central nervous system tumors. One of the widely used chemical agents for the treatment of glioblastoma is temozolomide. It is an orally administered, deoxyribonucleic acid (DNA) alkylating agent. DNA alkylation triggers the death of tumor cells. However, some tumor cells are able to repair this type of DNA damage and thus lower the therapeutic effect of temozolomide. Laboratory and clinical studies indicate that temozolomide's anticancer effects might be strengthened when combined with other chemotherapeutic agents like etoposide or antioxidant agents like ascorbic acid. In this study, we aimed to evaluate the cytotoxic and oxidative stress effects of ascorbic acid (1000 µM), temozolomide (100 µM) and etoposide (25 µM) agents alone and in dual and triple combinations in a glioblastoma U87 MG cell culture. mATERIAl and mEThODS: The cytotoxic and oxidative stress effects were investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) and liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis methods. RESulTS: Cytotoxicity tests showed that etoposide, temozolomide, "etoposide+ascorbic acid", "temozolomide+ascorbic acid", "temozolomide+etoposide" and "temozolomide+etoposide+ascorbic acid" combinations have anti-proliferative effects. The maximum anti-proliferation response was observed in the "temozolomide+etoposide+ascorbic acid"-added group. Similarly LCMS/MS analyses showed that minimum oxidative DNA damage occurred in the "temozolomide+etoposide+ascorbic acid"-added group. CONCluSION: Ascorbic acid decreases the cytotoxic and genotoxic effect of etoposide and etoposide-temozolomide combination but it has no meaningful effect on temozolomide's toxicity.