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    Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor
    (Springer, 2024) Kibar, Güneş; Şahinoğlu, O. Berkay; Kılınçlı, Betül; Erdem, E. Yegan; Çetin, Barbaros; Özalp, V. Cengiz
    This study introduces aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for adenosine triphosphate (ATP) detection where the POSS nanoparticles were synthesized in a one-step, continuous flow microfluidic reactor utilizing thermal polymerization. A microemulsion containing POSS monomers was generated in the microfluidic reactor which was designed to prevent clogging by using a continuous oil flow around the emulsion during thermal polymerization. Surfaces of POSS nanoparticles were biomimetically modified by polydopamine. The aptamer sequence for ATP was successfully attached to POSS nanoparticles. The aptamer-modified POSS nanoparticles were tested for affinity-based biosensor applications using ATP as a model molecule. The nanoparticles were able to capture ATP molecules successfully with an affinity constant of 46.5 ?M. Based on this result, it was shown, for the first time, that microfluidic synthesis of POSS nanoparticles can be utilized in designing aptamer-functionalized nanosystems for biosensor applications. The integration of POSS in biosensing technologies not only exemplifies the versatility and efficacy of these nanoparticles but also marks a significant contribution to the field of biorecognition and sample preparation. © The Author(s) 2024.
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    One-pot synthesis of organic-inorganic hybrid polyhedral oligomeric silsesquioxane microparticles in a double-zone temperature controlled microfluidic reactor
    (Wiley, 2019) Kibar, Gunes; Caliskan, Umutcan; Erdem, E. Yegan; Cetin, Barbaros
    Polyhedral oligomeric silsesquioxane (POSS) particles are one of the smallest organosilica nano-cage structures with high multifunctionality that show both organic and inorganic properties. Until now poly(POSS) structures have been synthesized from beginning with a methacryl-POSS monomer in free-radical mechanism with batch-wise methods that use sacrificial templates or additional multisteps. This study introduces a novel one-pot synthesis inside a continuous flow, double temperature zone microfluidic reactor where the methodology is based on dispersion polymerization. As a result, spherical monodisperse POSS microparticles were obtained and characterized to determine their morphology, surface chemical structure, and thermal behavior by SEM, FTIR, and TGA, respectively. These results were also compared and reported with the outcomes of batch-wise synthesis. (c) 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 1396-1403
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    Single, binary and successive patterning of charged nanoparticles by electrophoretic deposition
    (Springer Science and Business Media B.V., 2021) Sopubekova, Eliza; Kibar, Güneş; Erdem, E. Yegan
    Deposition of nanoparticles on a substrate in a controlled manner leads to the formation of multifunctional surfaces and therefore devices. Electrostatic forces can be utilized to manipulate different types of materials such as magnetic, insulating, conducting, semiconducting, organic and inorganic, without altering the chemistry of the surface. However, simultaneous and successive electrophoretic deposition (EPD) methods are not fully utilized for nanoparticles with different characteristics. In this work, electrostatic forces are applied to direct and position charged nanoparticles suspended in aqueous dispersions on desired areas of the surface. Assemblies of particles are obtained by electrostatic attraction generated by gold electrodes of sizes from 500 nm to 50 µm that are fabricated by thermal evaporation. Different types of charged nanoparticles were simultaneously attracted towards different locations of the surface by means of EPD; as a result, alternating nanoparticle patterns and particle deposition on the same designated areas for forming composite areas are obtained. Assemblies formed from positively charged silver nanoparticles and negatively charged fluorescent latex and silica nanoparticles are demonstrated. The position of metallic-, polymeric- and inorganic-based nanoparticles is controlled by the design of electrode geometry. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.