Yazar "Arisoy, Piril" seçeneğine göre listele

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    Öğe
    Advancing wound healing: controlled release of tannic acid via epitope imprinted antimicrobial spongy cover material
    (Springer, 2025) Tuna, Busra; Arisoy, Piril; Oktay Basegmez, Hatice Imge; Pesint, Gozde Baydemir
    The increasing resistance of microorganisms to conventional antibiotics calls for alternative antimicrobial strategies. This study introduces a novel approach to acute wound healing by incorporating epitope-imprinted spongy cover materials with antimicrobial properties, using Tannic acid (TA) as the active agent within biocompatible cryogels imprinted with gallic acid. The spongy materials were synthesized and characterized through Fourier Transform Infrared Spectroscopy (FTIR), swelling tests, and Scanning Electron Microscopy (SEM) to assess their structural and physicochemical properties. The antimicrobial efficacy of the cryogels, loaded with 1.5, 3, 5 mg/mL of TA concentrations, was tested against Staphylococcus aureus and Escherichia coli, common pathogens in wound infections. The highest inhibition zone was determined to be 15 mm for S. aureus and 12 mm for E. coli. Maximum TA adsorption was 210.27 mg/g for eMIP and 24.74 mg/g for NIP. Cumulative release studies revealed the highest release rate occurred within the first 2 h. TA release kinetics indicated a non-Fickian diffusion mechanism. Additionally, the biocompatibility and potential cytotoxicity of the spongy materials, including TA-loaded variants, were assessed using the MTT assay on cultured cells. The results confirmed that the spongy materials are non-toxic and do not inhibit cell proliferation, supporting their suitability for acute wound healing. This study demonstrates that TA-loaded epitope-imprinted Poly(2-hydroxyethyl methacrylate) (pHEMA)-based spongy materials possess antimicrobial properties, making them potential candidates for wound and burn dressing applications.
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    Öğe
    Development of molecularly imprinted nanoparticles for the detection of cardiovascular diseases biomarker Angiotensin II in human serum
    (Wiley, 2025) Arisoy, Piril; Pesint, Gozde Baydemir
    Angiotensin II (Ang II) is a peptide hormone that causes vasoconstriction and an increase in blood pressure. Due to its relationship with cardiovascular diseases, it is an important biomarker in blood serum. In this study, Ang II imprinted nanoparticles were synthesized by miniemulsion polymerization reaction for the determination of Ang II from human serum. Hydroxyethyl methacrylate (HEMA) based Ang II imprinted (Ang II-MIPnp) and non-imprinted nanoparticles (NIPnp) were synthesized, characterized by zeta size analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR-ATR). The average particle size of the NPs was recorded as 50 nm. Ang II molecules were successfully removed from the Ang II-MIPnp with a 98% success rate using 0.5 M NaCl solution to obtain template-specific cavities. Then, the adsorption studies were achieved. The binding capacity was found as 4500 pg. g(-1) at 700 pg. mL(-1) Ang II concentration. The selectivity studies showed that Ang II-MIPnp can recognize Ang II molecules 2.76 times and 3.23 times selectivity than Ang I and Vsp respectively. Reusability studies shows that the synthesized nanomaterial is reusable.
  • [ X ]
    Öğe
    Development of molecularly imprinted nanoparticles for the detection of cardiovascular diseases biomarker Angiotensin II in human serum
    (Wiley, 2025) Arisoy, Piril; Pesint, Gozde Baydemir
    Angiotensin II (Ang II) is a peptide hormone that causes vasoconstriction and an increase in blood pressure. Due to its relationship with cardiovascular diseases, it is an important biomarker in blood serum. In this study, Ang II imprinted nanoparticles were synthesized by miniemulsion polymerization reaction for the determination of Ang II from human serum. Hydroxyethyl methacrylate (HEMA) based Ang II imprinted (Ang II-MIPnp) and non-imprinted nanoparticles (NIPnp) were synthesized, characterized by zeta size analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR-ATR). The average particle size of the NPs was recorded as 50 nm. Ang II molecules were successfully removed from the Ang II-MIPnp with a 98% success rate using 0.5 M NaCl solution to obtain template-specific cavities. Then, the adsorption studies were achieved. The binding capacity was found as 4500 pg. g(-1) at 700 pg. mL(-1) Ang II concentration. The selectivity studies showed that Ang II-MIPnp can recognize Ang II molecules 2.76 times and 3.23 times selectivity than Ang I and Vsp respectively. Reusability studies shows that the synthesized nanomaterial is reusable.