Yazar "Annisa, Milkyas Endale" seçeneğine göre listele

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    Antibacterial and Antioxidant Activity of Isoflavans from the Roots of Rhynchosia ferruginea and In Silico Study on DNA Gyrase and Human Peroxiredoxin
    (2021) Hussien, Kalid; Eswaramoorthy, Rajalakshmanan; Melaku, Yadessa; Annisa, Milkyas Endale
    Rhynchosia ferruginea (Udusalim, Afan Oromo) is a medicinal plant traditionally used to treat skin infection, intestinal problems and amoebiasis. Silica gel chromatographic separation of dichloromethane/methanol (1:1) roots extract yielded isoflavan (1), isoflavene (2) and 1, 3-dilinoleoyl-2- stearoylglycerol (3), reported herein for the first time from the genus. Antibacterial activity was examined using disc diffusion method against E. coli, S. aureus, P. aeruginosa and S. pyogenes. AutoDoc vina 4.2 software was used for molecular docking analysis of compounds against human peroxiredoxin 5 and DNA gyrase B enzymes. Isoflavan (1) displayed zone of inhibition of 9.67 ± 0.58 mm and 10.67 ± 0.58 mm whereas isoflavene (2) showed 10.33 ± 1.15 mm and 10 ± 1.00 mm against E. coli and S. aureus, respectively, compared to ciprofloxacin (15.67±0.58 mm for both strains). DPPH radical scavenging assay of the dichloromethane/methanol (1:1) roots extract and isoflavan (1) exhibited better radical scavenging activity with $IC_{50}$ value of 17.7 and 32, respectively. Molecular docking analysis revealed that 1 and 2 exhibited similar binding affinity of -7.4 kcal/mol compared to ciprofloxacin (-7.3 kcal/mol). In silico analysis against human peroxiredoxin 5 (PDB ID: 1HD2) revealed minimum binding affinity of - 3.7 and - 2.0 kcal/mol for compounds 1 and 2, respectively, compared to ascorbic acid (-4.9 kcal/mol). The in vitro antibacterial and antioxidant activity of compounds 1 and 2 suggest the potential use of these compounds as drug lead candidates which corroborate with the traditional uses of the roots of R. ferruginea.
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    Chemical Composition, Pharmacological Activities and Biofuel Production of Eichhornia crassipes (Water Hyacinth): A Review
    (2022) Gebrehiwot, Hadush; Dekebo, Aman; Annisa, Milkyas Endale
    Eichhornia crassipes is one of the free floating macrophytes with substantial damaging effects on aquatic environment and economic development. It is listed as one of the most productive plants on earth and among the top ten world’s worst aquatic plants. Several metabolites such as vitamins, terpenoids, phenolic acids, lignins, stilbens, alkaloids and sterols have been reported from the plant. This review endeavors to provide a comprehensive and up to date compilation of documented chemical constituents, pharmacological activities and renewable energy profile of water hyacinth. The plant demonstrated wide spectrum of uses including biofuel production, medicinal function, vermicomposting, compost production and bioremediation. Anti-inflammatory, antioxidant, antifungal, antiaging, DPPH radical scavenging, hepatoprotective and antibacterial functions are well documented medicinal uses of the plant of which tremendous antioxidant and antibacterial activities were noticed. On the other hand, clogging of waterways, breeding grounds for pests and disease, reduction of water quality, loss of biodiversity and economic recession in invaded areas are negative aspects associated with it. The present review therefore discusses the chemical makeups, pharmacological and renewable energy aspects of the weed.
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    Pharmacokinetics, drug-likeness, antibacterial and antioxidant activity of secondary metabolites from the roots extracts of Crinum abyssinicum and Calotropis procera and in silico molecular docking study
    (2022) Tegegn, Getachew; Melaku, Yadessa; Eswaramoorthy, Rajalakshmanan; Annisa, Milkyas Endale
    Crinum abyssinicum and Calotropis procera were traditionally used for the treatment of different diseases such as hypertension, diabetes, hepatitis B, skin infection, anticancer, asthma, fever, and diarrhea. The structures of the compounds were characterized by 1H NMR, 13C NMR, and DEPT-135 spectra. Compounds 1-3 were reported herein for the first time from the species of C. abyssinicum. The DCM/MeOH (1:1) and MeOH roots extracts of C. abyssinicum showed significant inhibitory activity against S. aureus and P. aeruginosa with a mean inhibition zone of 16.67 ± 1.20 and 16.33 ± 0.33 mm, respectively. Compounds 4 and 5 showed promising activity against E. coli with a mean inhibition zone of 17.7 ? 0.8 and 17.7 ? 1.2 mm, respectively. The results of DPPH activity showed the DCM: MeOH (1:1) and MeOH roots extracts of C. abyssinicum inhibited the DPPH radical by 52.86 ? 0.24 % and 45.6 ? 0.11 %, respectively, whereas compound 5 displayed 85.7 % of inhibition. The drug-likeness analysis showed that compounds 2-4 satisfy Lipinski’s rule of five with zero violations. Compounds 2, and 6 showed binding affinities of ?6.0, and ?6.7 kcal/mol against E. coli DNA gyrase B, respectively, while 3 and 5 showed ?5.0 and ?5.0 kcal/mol, respectively against human peroxiredoxin 5. Therefore, the in vitro antibacterial, radical scavenging activity along with the molecular docking analysis suggest the potential use of the extracts of C. abyssinicum and compounds 2, 5, 6, and 3, 5 can be considered as promising antibacterial agents and free radical scavengers, respectively.
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    Phytochemicals, Traditional Uses and Pharmacological Activity of Rhamnus prinoides: A Review
    (2021) Nigussie, Gashaw; Tola, Mekdes; Mohammed, Fozia; Werede, Yemane; Tegegn, Metasebia; Neway, Sebsib; Annisa, Milkyas Endale
    Rhamnus prinoides L’Herit belongs to Rhamnaceae family widely distributed in India, Eastern, Central and Southern Africa. In Ethiopia it is commonly known as Gesho (Amharic, Tigrigna and Afan Oromo), Gishe (Guragegna) and Geshu (Agewgna). Decoction of the leaves is recommended as remedy for treatment of variety of diseases such as back pain, malaria, pneumonia, sexually transmitted disease, skin infections, wounds, blood purifiers, water borne diseases and as ethnoveterinary medicine. Various secondary metabolites such as flavonoids, alkaloids, tannins, terpenoids, saponins, steroids and anthraquinones have been reported from the genus of which polyphenols were abundant with tremedousantioxidant, wound healing and antiinflammatory activities. The plant also serve as hopping agent, making traditional alcoholic beverages like tella and tej (in Ethiopia), animal feed, medicine, nectar for bees, soil conservation, ornamental, shade and dyes in textiles. This review presents chemical profile as well as biological activities of the species which confirmed that the plant is a good source natural polyphenols and provided valuable information in support of its use as an alternative medicine for future healthcare practice.
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    Traditional Medicinal Uses, Phytochemicals, and Pharmacological Activities of Genus Rhamnus: A review
    (2021) Nigussie, Gashaw; Melak, Haregua; Annisa, Milkyas Endale
    The genus Rhamnus belongs to the Rhamnaceae family, which contains approximately 137species, traditionally used as folk medicine in East Asia, North and South America, and subtropicalregions of Africa. The genus is used traditionally to treat diseases such as cancer, wound, jaundice,hepatitis, gonorrhea, laxative, hypertension, malaria, stomach ache, snake bite and diarrhea.Anthraquinones and flavonoids are the most cited compounds from the genus of which polyphenols wereabundant with tremendous antioxidant, wound healing and antiinflammatory activities. Pharmacologicalactivity evaluation of the extracts and isolated compounds revealed anti-inflammatory, antioxidant,antimalarial, antibacterial, anti-mutagenic, anti-genotoxic, hepatoprotective, anticancer, and antiproliferative activity. The genus afforded drug leads such as 6-methoxysorigenin (12) and prinoidin (23)with anti-tyrosinase and cytotoxicity, respectively, as well as antioxidant drug leads such as Kaempferol3-O-?-rhamninoside (31) rhamnetin-3-O-?-isorhamninoside (37) and isotorachrysone (55). Thepresent review endeavors to provide a comprehensive and up to date compilation of documentedtraditional medicinal uses, phytochemicals and pharmacological activities of the genus and providedvaluable information in support of its uses as an alternative medicine for future healthcare practice.