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Öğe Characterization of Aroma-Active Compounds in Seed Extract of Black Cumin (Nigella sativa L.) by Aroma Extract Dilution Analysis(Mdpi, 2018) Kesen, Songul; Amanpour, Armin; Sarhir, Salwa Tsouli; Sevindik, Onur; Güçlü, Gamze; Kelebek, Haşim; Selli, SerkanTurkish Nigella sativa L. seed extracts were used to detect the aroma and key odorant compounds of the spice using gas chromatography-mass spectrometry-olfactometry (GC-MS-O). Volatile compounds were extracted by the purge and trap extraction (PTE) method. A total of 32 volatile compounds consisting of different chemical classes acids (13), alcohols (7), phenols (3), terpene (1), esters (2), ketones (2), aldehyde (1), lactone (1) and hydrocarbons (2) were determined. The amounts of volatile compounds were found to be 21,544 mu g kg(-1). The application of aroma extract dilution analysis (AEDA) revealed the presence of 13 odor-active compounds alcohols (2), carboxylic acids (4), phenols (2), terpene (1), ketone (1), hydrocarbon (1) and unknown compounds (2) in Nigella sativa L. extract. Flavor dilution (FD) factors of key odorants ranged between 4 and 1024, while odor activity values (OAV) were in the range of 1.0 to 170.8. Acetoin was the only aroma-active ketone detected in Nigella sativa L. seed extracts. It had the strongest aroma (FD = 1024) and provided a buttery odor. This compound represented the most abundant compound of overall aroma profile with a concentration of 9394 mu g kg(-1), followed by isobutanoic acid (FD = 512 with a concentration of 218 mu g kg(-1)) and contributed a powerful aroma and a cheesy characteristic odor.Öğe Characterization of key aroma compounds in fresh and roasted terebinth fruits using aroma extract dilution analysis and GC-MS-Olfactometry(Elsevier, 2019) Amanpour, Armin; Güçlü, Gamze; Kelebek, Haşim; Selli, SerkanAroma, key aroma compounds, and their odor activity values of fresh and roasted P. terebinthus fruits were studied. Aroma compounds were extracted by the solvent-assisted flavor evaporation technique and analyzed by GC-MS-Olfactometry for the first time. A total of 64 different aroma compounds were detected (57 in fresh and 62 in roasted samples), including terpenes (34), acids (8), aldehydes (4), ketones (4), pyrazines (5), alcohols (5), pyrrole (1), furan (1), lactone (1) and ester (1). Terpenes were the predominant group followed by acids. Aroma profiles of both samples were quite analogous. Nonetheless, pinocarvone and dodecanoic acid were detected only in fresh fruits, while pyrazine class, 5-methylfurfural and gamma-butyrolactone were emerged only in roasted fruits. Aroma extract dilution analysis (AEDA) was applied for the first time and revealed 31 key aroma compounds. Among them, the most powerful key aroma compounds with the greatest FD factors were alpha-pinene and beta-myrcene.Öğe GC-MS-olfactometric characterization of the most aroma-active components in a representative aromatic extract from Iranian saffron (Crocus sativus L)(Elsevier Sci Ltd, 2015) Amanpour, Armin; Sönmezdağ, A. Salih; Kelebek, Haşim; Selli, SerkanAroma and aroma-active compounds of Iranian saffron (Crocus sativus L.) were analyzed by gas chromatography-mass spectrometry-olfactometry. The saffron aromatic extracts were obtained by four different extraction techniques including solvent-assisted flavour evaporation (SAFE), liquid-liquid extraction (LLE), solid phase extraction (SPE), and simultaneous distillation extraction (SDE) and compared to achieve a representative aromatic extract from saffron. According to sensory analysis, the aromatic extract obtained by SAFE was the most representative of saffron odour. A total of 28 aroma compounds were identified in saffron. Ketones were quantitatively the most dominant volatiles in saffron, followed by aldehydes and acids. Aroma extract dilution analysis (AEDA) was used for the determination of aroma-active compounds of saffron. A total of nine aroma-active compounds were detected in the aromatic extract. On the basis of the flavour dilution (FD) factor, the most powerful aroma active compounds were safranal (FD = 512), 4-ketoisophorone (FD = 256) and dihydrooxophorone (FD = 128). (C) 20115 Elsevier Ltd. All rights reserved.Öğe Non-thermal plasma effects on the lipoxygenase enzyme activity, aroma and phenolic profiles of olive oil(Elsevier Sci Ltd, 2019) Amanpour, Armin; Vandamme, Jeroen; Polat, Suleyman; Kelebek, Haşim; Van Durme, Jim; Selli, SerkanEffects of non-thermal plasma (NTP) technology on the aroma and phenolic compositions and inactivation of lipoxygenase (LOX) enzyme activity of extra virgin olive oil were investigated for the first time. A short non thermal plasma treatment with pure argon gas (2 standard liters per minute and an average voltage of 7 kV) was applied for 135 s on the olive oil sample. The LOX activity in the NTP treated oil was inhibited up to 42.9% in comparison with untreated oil. Mean values of the peroxides in both samples, expressed in meq oxygen/kg of oil, did not show any statistically significant difference. The values of the antioxidant potentials in the samples proved a mild reduction after NTP treatment. A total of 14 phenols and 31 volatiles were found in both samples with same profile. Although there was a slight difference in the test samples with regard to the concentration of individual phenolic and aroma compounds, this difference was not statistically significant. Therefore, NTP treatment had no statistically significant influence on the antioxidant activity, peroxide value, phenols and volatiles, except LOX activity.Öğe The most aroma-active compounds in shade-dried aerial parts of basil obtained from Iran and Turkey(Elsevier, 2018) Sönmezdağ, Ahmet Salih; Amanpour, Armin; Kelebek, Haşim; Selli, SerkanVolatile profile, aroma-active compounds and odor activity values of the shade-dried aerial parts of basil (Ocimum basilicum) were investigated. Basil samples used under the study were provided from Iran and Turkey. Volatile compounds were isolated using a purge and trap extraction system and analyzed by gas chromatography olfactometry. A total of 50 volatile compounds of which 29 originated from Iranian and 32 were of Turkish origin were determined. Terpenes were present at the overwhelmingly highest levels, followed by alcohols and aldehydes. Of the terpenes, methyl chavicol was the main compound of both samples. The aroma-active compounds of basils were investigated by using aroma extract dilution analysis (AEDA) for the first time. The application of AEDA revealed 18 aroma-active compounds, including terpenes (10), aldehydes (3), ketone (1), phenol (1), alcohol (1), and unknown compounds (2) were detected. Linalool and methyl chavicol had the greatest flavour dilution (FD) factors in both samples, amounting to 2048 and 1024, respectively.