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    Comparative Study of Pectin and Kappa-Carrageenan-Based Films Loaded With Boswellic Acid and Acetyl-11-Keto-Beta-Boswellic Acid for Active Packaging
    (Wiley, 2025) Shah, Yasir Abbas; Bhatia, Saurabh; Al-Harrasi, Ahmed; Tarahi, Mohammad; Khan, Talha Shireen; Alam, Tanveer; Koca, Esra; Aydemir, Levent Yurdaer
    Active packaging is crucial for extending shelf life, maintaining food quality, and reducing reliance on synthetic preservatives by preventing spoilage, oxidation, and microbial contamination. In this study, boswellic acid (BA) and acetyl-11-keto-beta-boswellic acid (AKBA) were isolated from the oleo-gum resin of Boswellia sacra and incorporated into the pectin and kappa-carrageenan-based films for active packaging. The fabricated films were analyzed to examine the effect of these active compounds on the mechanical, optical, barrier, chemical, structural, hydrophobic, and antioxidant properties. The incorporation of AKBA and BA into the films resulted in a decrease in transparency and tensile strength; however, the elongation at break did not show any significant variations. The film samples exhibited improved antioxidant properties as confirmed by DPPH and ABTS free radical scavenging activities. The Delta E value represented the overall color difference in AKBA- and BA-loaded films as compared with the blank film sample. The FTIR spectrum of AKBA- and BA-loaded films exhibited shifts and intensity changes, suggesting the intermolecular interaction within the film matrix. Moreover, the water contact angle measurements revealed that the films containing AKBA showed a noticeable increase in contact angles, reflecting an enhancement in hydrophobicity. The current study offers a valuable approach for developing active packaging films with potential applications in food packaging.
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    Development of Peru balsam-enriched pectin-gellan gum films for sustainable antioxidant food packaging
    (Springer, 2025) Bhatia, Saurabh; Alhadhrami, Aysha Salim; Jawad, Muhammad; Chinnam, Sampath; Shah, Yasir Abbas; Al-Harrasi, Ahmed; Koca, Esra; Aydemir, Levent Yurdaer; Alam, Tanveer; Mohan, Syam; Zoghebi, Khalid; Khalid, Asaad
    In this study, the effects of Perubalsam extract (PBE) on the surface, physical, mechanical, barrier and antioxidant properties of pectin/Gelrite gellen gum (PGG) were evaluated. The developed films were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), derivative thermogravimetry (DTG), and X-ray diffraction (XRD). Moreover, physiochemical properties, including moisture content, color, opacity, water solubility, and water vapor permeability, were measured. The antioxidant activity of the prepared films was determined by DPPH and ABTS radical scavenging assays. The incorporation of the extract into films increased opacity, thickness, elongation at break, water vapor permeability, and water solubility; however, tensile strength and moisture content decreased. SEM analysis revealed microstructural deformities like pores and cracks at higher concentrations of PBE. As expected, the antioxidant activities of the composite films increased with the increase in the PBE concentration in the films. Thus, such natural extract antioxidant films as a sustainable packaging material could offer better protection to the packed product against undesirable oxidation reactions.
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    Effect of Pinus radiata extract on the properties and performance of sodium alginate and kappa-carrageenan composite films
    (Springer, 2025) Bhatia, Saurabh; Jawad, Muhammad; Shah, Yasir Abbas; Chinnam, Sampath; Al-Harrasi, Ahmed; Koca, Esra; Aydemir, Levent Yurdaer; Alam, Tanveer; Mohan, Syam; Zoghebi, Khalid; Khalid, Asaad
    Composite films of sodium alginate and kappa carrageenan (SAC) enriched with Pinus radiata bark extract (EnPBE) were fabricated using the casting method. The effects of varying EnPBE concentrations (0.1-1% v/v) on the SAC-based films were comprehensively evaluated. Assessments included surface color measurement, surface hydrophobicity, atomic force microscopy, transparency analysis, barrier properties, X-ray diffraction, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy and antioxidant analysis. Thermal properties were analyzed using thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Additionally, in-vitro release studies were performed to study the release behavior of the EnPBE. The findings revealed that increasing the EnPBE concentration led to a notable decrease in the swelling index and film transparency, while thickness, moisture content, water permeation, and flexibility increased. Color analysis revealed a significant increase in the red/green value (a*) and yellow/blue value (b*) of the films, consistent with visual observations. The water contact angle values decreased from 49.06 degrees to 41.45 degrees with the addition of EnPBE. Moreover, the release of the extract in both aqueous and methanolic environments showed a significant increase at higher concentrations. DPPH and ABTS antioxidant assays demonstrated a concentration-dependent increase in the free radical/cation scavenging capacity of the films. The surface roughness of the films increased with the addition of EnPBE, consistent with SEM results that showed particles on the surface of EnPBE-loaded films. TGA and DSC analyses demonstrated improved thermal stability of the films with the incorporation of EnPBE.
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    Exploring the impact of Galbanum on the functionality of gelatin-based packaging films
    (Frontiers Media SA, 2026) Bhatia, Saurabh; Shah, Yasir Abbas; Alhadhrami, Aysha Salim; Khan, Talha Shireen; Jawad, Muhammad; Al-Harrasi, Ahmed; Koca, Esra; Aydemir, Levent Yurdaer; Alam, Tanveer
    Introduction The current study was designed to investigate the effect of Galbanum absolute over the physico-chemical and antioxidant properties of gelatin films.Methods Traditional casting method was used to fabricated gelatin and galbanum absolute film samples. The developed films were further subjected to FTIR, XRD, SEM, and DSC analysis determine their physiochemical and structural properties.Results It was found that with an increase in the concentration of Galbanum absolute, film thickness (0.07 mm - 0.17 mm), thermal stability, and contact angle (51.53 degrees - 80.14 degrees) were increased whereas transmittance (63.44 - 90.32) and crystallinity of the samples decreased. Furthermore, the incorporation of Galbanum absolute significantly increased the antioxidant properties (from 39.44% to 61.99% for DPPH radical scavenging activity and 8.89% to 62.86% for ABTS radical scavenging activity) of gelatin films. However, micrographs of Galbanum absolute-loaded films showed an increase in roughness and cracks over the films with the addition of Galbanum absolute.Discussion Therefore, further optimization of the Galbanum absolute proportion in the biopolymer films can result in the development of stable active packaging. These findings highlight the potential applications of Galbanum absolute as a natural additive in biodegradable food packaging materials to enhance the thermal stability and antioxidant properties.
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    Extraction and applications of frankincense oleoresin as functional ingredient in pectin/sodium-alginate composite films for active packaging
    (Elsevier, 2025) Shah, Yasir Abbas; Bhatia, Saurabh; Al-Harrasi, Ahmed; Tarahi, Mohammad; Jawad, Muhammad; Alam, Tanveer; Diblan, Sevgin; Koca, Esra; Aydemir, Levent Yurdaer; Thekkuden, Dinu Thomas; Imam, Faisal; Al-Harbi, Naif
    The composite films fabricated by incorporating frankincense oleoresin (FOR) into pectin and sodium alginate matrices, were underwent several analysis including thickness, mechanical strength, barrier properties, color attributes, morphological structure, surface roughness, chemical composition, crystalline nature, as well as evaluations of antioxidant and antimicrobial properties. The findings of the study indicated an increase in the thickness of the film samples with the addition of FOR however reduction in both tensile strength (from 9.59 to 2.58 MPa) and elongation at break (from 9.85 % to 3.22 %) was observed. The water vapor permeability of the developed films demonstrated an increase from 0.411 to 0.878 (g*mm)/(m2*h*kPa). Atomic Force Microscopy (AFM) imaging results indicated a reduction in the surface roughness of films upon the addition of FOR. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) spectra unveiled chemical interactions between the film-forming polymers and FOR. Furthermore, a significant enhancement in the antioxidant activity was observed in films incorporating FOR, compared to the control group. These FOR-loaded film samples demonstrated antimicrobial effects against Pseudomonas aeruginosa, while no inhibitory zones were observed against Staphylococcus aureus and Candida albicans. In summary, the outcomes of this investigation highlight the promising potential of pectin/sodium alginate composite films containing FOR in packaging applications.
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    Extraction and Applications of Oakmoss Absolute Extract as a Functional Ingredient in Alginate-Guar Gum Composite Films for Food Packaging
    (Wiley, 2025) Bhatia, Saurabh; Alhadhrami, Aysha Salim; Shah, Yasir Abbas; Jawad, Muhammad; Koca, Esra; Aydemir, Levent Yurdaer; Alam, Tanveer; Al-Harrasi, Ahmed; Anwer, Md. Khalid; Mohan, Syam; Najmi, Asim; Khalid, Asaad
    Oakmoss absolute extract (OME) is a potential source for active antimicrobial and antioxidant components that can be used to develop an active packaging material. Active films were developed by impregnation of OME in sodium alginate (SA) and guar gum (GG)-based films. The effects of 0.5%-1.5% OME (w/v) over the physicochemical and antioxidant properties of SA-GG films were assessed. The mechanical properties (TS, EAB), water permeability, thickness, and opacity increased; however, moisture content decreased with the increase in the concentration of OME. SEM analysis represents that incorporation of OME resulted into heterogenous structure with pores and cracks. FTIR analysis showed that the addition of OME into SA-GG triggered interactions between SA-GG and OME. The color attributes of SA-GG films were significantly affected by the incorporation of OME. The XRD analysis revealed that the amorphous nature of the film remained unchanged after impregnation with OME. The films loaded with OME demonstrated higher antioxidant activity (measured by DPPH as well as ABTS radical scavenging) than control film. Thus, the addition of OME into SA-GG film was a valuable approach to synthesize active, bio-functional, and eco-friendly packing for the food industry.
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    Myrrh Oleo-Gum Resin as a Functional Additive in Pectin and ?-Carrageenan Composite Films for Food Packaging
    (Wiley, 2024) Shah, Yasir Abbas; Bhatia, Saurabh; Chinnam, Sampath; Al-Harrasi, Ahmed; Tarahi, Mohammad; Khan, Talha Shireen; Alam, Tanveer
    Myrrh oleo-gum-resin (MOGR) is a natural substance that has a rich history of medicinal use due to its anti-inflammatory, antimicrobial, and antioxidant properties. The present study reports on the fabrication and assessment of pectin and K-carrageenan composite films infused with varying proportions (0.3%, 0.5%, and 0.7%) of MOGR. Morphological analysis of the film samples was conducted using Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The results indicated that the introduction of MOGR led to a notable increase in surface roughness. The SEM micrographs of the films showed that the MOGR addition had an important effect on the microstructure of the film. The surface hydrophobicity of the MOGR-loaded films increased, as confirmed by the rise in the contact angle. Moreover, there was an increase in the thickness (0.062 +/- 0.004-0.095 +/- 0.006 mm) and opacity (1.24 +/- 0.07-9.41 +/- 0.24) of the films with the addition of MOGR; however, tensile strength (7.30 +/- 0.50-4.92 +/- 0.34 MPa), elongation at break (32.41% +/- 1.0%-29.70% +/- 0.24%), and barrier properties decreased. Additionally, a rise in MOGR concentration corresponded to a rise in overall color difference Delta E (0.77 +/- 0.03-5.09 +/- 0.49) of the films. Notably, the incorporation of MOGR led to an increase in the antioxidant activity of the composite films, indicating potential applications in functional packaging materials.
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    Pectin/sodium alginate films tailored with Acetyl-11-keto-beta-boswellic acid for active packaging
    (Elsevier, 2024) Bhatia, Saurabh; Shah, Yasir Abbas; Al-Harrasi, Ahmed; Jawad, Muhammad; Khan, Talha Shireen; Alam, Tanveer; Diblan, Sevgin
    The present study aimed to develop food packaging films by using a combination of pectin (PE) and sodium alginate (SA) enriched with Acetyl-11-keto-beta-boswellic acid (AKBA) as a functional or active ingredient. The fabricated films underwent comprehensive evaluation of their morphological, chemical, mechanical, barrier, optical, thermal, antioxidant, and antimicrobial properties. SEM and FTIR analysis showed that AKBA had good compatibility with film-forming components. The AKBA-loaded film samples exhibited a decrease in their barrier properties and tensile strength, but enhancements in both elongation at break and thickness values was observed. With the addition of AKBA, a significant increase (p < 0.05) in the ultraviolet barrier properties of the films and total colour variation (Delta E) was observed. TGA analysis of the films unveiled an improvement in thermal resistance with the incorporation of AKBA. Moreover, the films loaded with AKBA exhibited potent antioxidant activity in the ABTS and DPPH assay methods. Disk diffusion analysis showed the antimicrobial activity of AKBAloaded films against P. aeruginosa, highlighting the potential of AKBA as a natural antimicrobial agent for the safety of food products. The results demonstrate the practical application of PE and SA active films loaded with AKBA, particularly within the food packaging industry.