Mohsenzadeh, ElhamDemir, DidemCeylan, SedaKhenoussi, NabylSchacher, LaurenceAdolphe, DominiqueBolgen, Nimet2025-01-062025-01-0620230250-47070973-766910.1007/s12034-023-02975-72-s2.0-85162693310https://doi.org/10.1007/s12034-023-02975-7https://hdl.handle.net/20.500.14669/3118In this study, a bi-layer scaffold combining polyurethane nanofibrous and silicone membrane layers was produced. Chemical, morphological and physical properties of the scaffolds were determined by Fourier-transform infrared spectroscopy, scanning electron microscope (SEM) and Brunauer-Emmett-Teller analyses, respectively. The surface properties were examined with the contact angle test. To evaluate the encapsulation and release behaviour of the scaffolds Rhodamine B and Nile red were used as model drugs. Further, the cytotoxicity and cell proliferation investigations were carried out using mouse embryonic fibroblasts cell lines. 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide assay and SEM were used to investigate the cell viability and cell-scaffold interactions, respectively. The results of the study were evaluated in order to develop a bimodal drug release system that has the potential to be used in tissue engineering applications.eninfo:eu-repo/semantics/closedAccessTissue engineeringelectrospinningbi-layer scaffoldpolyurethanesiliconArticle3Q246WOS:001012467800003Q3