Kucuk, DilrubaUner, GuelcanIpek, Semih LatifCaglayan, Mustafa OguzhanUstundag, Zafer2025-01-062025-01-0620240041-01011879-315010.1016/j.toxicon.2024.1081152-s2.0-85205436006https://doi.org/10.1016/j.toxicon.2024.108115https://hdl.handle.net/20.500.14669/3210Zearalenone (ZEN) is a mycotoxin that poses significant risks to human and animal health due to its mutagenic, immunosuppressive, and carcinogenic properties. This study presents a novel analytical method for detecting ZEN using electrochemical impedance spectroscopy (EIS) combined with a molecularly imprinted polymer (MIP). ZEN, used as the template molecule, was incorporated into polypyrrole on screen-printed electrodes (SPE), and a ZEN-sensitive MIP sensor was created through template removal. The modified sensor surfaces were characterized by EIS and scanning electron microscopy (SEM). An impedimetric MIP sensor for ZEN was developed, offering a detection range from 1 pM to 500 pM. The method's limit of detection (LOD) was established at 1 pM (0.3 pg/mL) with a signal-to-noise ratio of 3 (S/N = 3). The method demonstrated high precision and accuracy, with a maximum relative standard deviation (RSD) of less than 4.4% at a 95% confidence level, and relative error (RE) values ranging from -0.8% to -2.7%. The selectivity of the developed MIP sensor was evaluated using ochratoxin A, ochratoxin B, and aflatoxin B1, with no significant interference observed. ZEN recovery from spiked samples was between 95% and 105%, indicating that the method was successfully applied to grain samples, including corn, rice, and wheat.eninfo:eu-repo/semantics/closedAccessZearalenoneMycotoxinElectrochemical impedance spectroscopyMolecularly imprinted polymerArticle39368557Q2250WOS:001333348200001N/A